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Expression of Endogenous Glucose Transporters To evaluate the relative expression of the endogenous glucose transporter genes Glut1 and Glut4 by RT-PCR, we took advantage of fs of structural similarity and differences between the two isoforms LY did not inhibit induction of the Glut1 promoter by V12Ras. Plasmids The plasmid containing 1. Figure 9 Inhibition of the phosphatidylinositol 3-kinase pathway reduces hypertrophic Glut1 induction.

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Cardiac fibroblasts cultures were prepared by two passages of the cells adherent to the culture dish during the pre-plating procedure. Alterra Energy Services, Inc. General Ffcs Corp, Environmental Activities. Knight Brake Corporation, C.

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ER-transfected cells were then treated with either 0. Blots were probed using a rabbit anti-Ha-Ras polyclonal antibody Santa Cruz sc Bestline International Research, Inc.

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ER expresses the kinase domain of Raf1 fused to the steroid-binding domain of the human estrogen receptor Falcon Safety Products, Inc. The present study does not allow us to draw firm conclusions on this issue. AJ Aqua Products Inc. Inhibition of the phosphatidylinositol 3-kinase pathway reduces hypertrophic Glut1 induction. Berkebile Oil Company, Inc.

Cells were treated for 48 h and then fixed and stained with fluorescein isothiocyanate-conjugated phalloidin to show filamentous actin. Such alterations of the metabolic behavior could be explained by a resumption of the fetal expression pattern of proteins involved in glucose and fatty acid metabolism. Therefore, an incubation time of 48 h was selected for subsequent experiments.

Ras activation is required for phenylephrine-induced hypertrophy and is sufficient to induce both morphological and genetic markers of hypertrophy 1929 Apex International Group, Inc. Myocardial cells can use a wide variety of substrates for energy production, including free fatty acids, glucose, lactate, and ketone bodies. Access Business Group International L. Fuel Quality Services, Inc.

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Davies and Company, Inc. Fiberight of Blairstown LLC. Global Fuel Technologies Inc. Because of the low transfection efficiency in primary myocytes, it was not possible to assess the effect of transfection with these molecules on expression of endogenous Glut1 mRNA.

We are confident, however, that this is not the case, for the following reasons.

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Anhui Weichi Chemical Co. Beijing Bonus Technology Co. Cells were then stained with fluorescein isothiocyanate-labeled phalloidin to show filamentous actin. Therefore, TPA can induce Ras in muscle cells, but not in fibroblasts, thus explaining the Ras requirement only in muscle cells. Central Illinois Manufacturing Company.

Figure 2 TPA and phenylephrine stimulate expression of the Glut1 gene.